UTEX LB 3174
Medlinella amphoroidea

Algae Details

UTEX Number: 3174
Class: Bacillariophyceae
Strain: Medlinella amphoroidea (Frankovich, Ashworth & M.J. Sullivan)
Medium: F/2 Medium (F/2)
Origin: Marathon, Florida, USA
Description of Location: Microscopic epiflora growing on the skin of the neck of a green sea turtle (Chelonia mydas) in a rehabilitation facility, collected via toothbrush.
GPS: 24.71, -81.10
Type Culture: No
Collection: Thomas Frankovich (03/23/2021)
Isolation: Matt Ashworth (03/26/2021)
Isolator Number: TH 23III21 Cm neck Kent MedlinellaTH124
Deposition: Matt Ashworth (09/21/2022)
Also Known As:
Notes: This is a raphid pennate diatom, <15 µm in length, <5 µm in width and <10 µm in pervalvar length. The valve is asymmetric along the longitudinal axis. The taxon is photosynthetic. Septa on the first two girdle elements can be seen on the frustule in girdle view.

Strains that are maintained only in cryopreservation (designated 'cryo only') require a minimum of 4-weeks to grow out before shipment.

Cryopreservation Conditions

Temperature: -190 °C
Storage: Vapor phase of liquid nitrogen dewars

Cryopreservation stabilizes genomic integrity, preserves culture quality, minimizes maintenance costs and reduces the risk of catastrophic loss. A broad variety of techniques employed at the CCAP, at UTEX, and elsewhere, can be found in Day, J.G. and Brand, J.J. Cryopreservation Methods for Maintaining Microalgal Cultures, in Algal Culturing Techniques, ed. R. A. Andersen, Elsevier, Amsterdam, 2006. A relatively simple method that uses a minimum of specialized equipment, yet allows a large variety of microalgae to be cryopreserved with high viability, can be found on the Cryopreservation of Microalgae page.

Preparation of Strains Maintained Only In Cryopreservation For Orders

Strains that are maintained only in cryopreservation (cryo only) require a minimum of 4-weeks to thaw, grow out, and check viability before shipment. An estimated shipping date will be emailed when purchased. Updates via email will be sent if the strain is ready sooner or needs to be delayed further.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

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