Trace Metals Solution Recipe


Trace Metals Solution Recipe

Directions

For 1 L Total


1. Begin stirring and heating approximately 950 mL of dH2O.
2. When the stirring water approaches the boiling point, add the following components in the order listed.
3. Bring the volume to 1 L with dH2O.
4. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 ZnSO47H2O (Sigma Z 0251) 23 mg/L 0.08 µM
2 MnSO4H2O (Sigma M8179) 152 mg/L 0.9 µM
3 Na2MoO42H2O (J.T. Baker 3764) 7.3 mg/L 0.03 µM
4 CoSO47H2O (Baker 1696) 14 mg/L 0.05 µM
5 CuCl22H2O (Fisher C-455) 6.8 mg/L 0.04 µM
6 Fe(NH4)2(SO4)26H2O (Sigma F-1513) 4.6 g/L 11.7 µM
7 Na2EDTA2H2O (Sigma ED255) 4.4 g/L 11.7 µM

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.