Spirulina Solution 2 Recipe


Spir solution 2 Recipe

Directions
For 500 mL Total

1.To approximately 450 mL of dH2O, add the following components (except vitamins) in the order listed while stirring continuously.
2. Bring total volume to 500 mL with dH2O.
3. Autoclave medium.
4. Add vitamin B12.

# Component Amount Stock Solution
Concentration
Final Concentration
1 NaNO3 (Fisher BP360-500) 2.5 g/500 mL 29.4 mM
2 K2SO4 (Sigma P 4042) 1 g/500 mL 5.74 mM
3 NaCl (Fisher S271-500) 1 g/500 mL 17.1 mM
4 MgSO47H2O (Sigma 230391) 0.2 g/500 mL 0.81 mM
5 CaCl22H2O (Sigma C-3881) 0.04 g/500 mL 0.27 mM
6 P-IV Metal Solution 6 mL/0.5 L
7 Chu Micronutrient Solution 1 mL/0.5 L
8 Vitamin B12 1 mL/0.5 L
UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.