Soilwater: GR+/NH4 Medium

Upcoming Order Deadlines:

Orders for algal culture media placed after 5:00 PM CST on Wednesday, November 14th through Wednesday, November 28th, will not ship until Monday, December 3rd, 2018. There will be no outgoing shipments on November 26th, 2018. Please view more information here.




Soilwater: GR+/NH4 Medium Recipe

The basic garden-type soilwater; includes a pinch of CaCO3, which is added to the soil and water prior to steaming; suitable for most phototrophic freshwater algae. Optional ingredients: add vitamin B12 to cultures of Volvox; a pinch of NH4MgPO46H2O added to soilwater cultures of Botryococcus, Synechococcus and some Euglenoids enhances growth and to LB 826 Gonium pectorale increases the numbers of 16-celled colonies formed.

Directions

For 200 mL Total

  1. Prepare Soilwater: GR+ Medium
  2. Add pinch of NH4MgPO4 after pasteurizing.

# Component Amount Stock Solution Concentration Final Concentration
1 Soilwater: GR+ Medium 200 mL
2 NH4MgPO46H2O (Sigma 529354) 1 mg 0.04 mM