P-IV Metal Solution Component Recipe


P-IV Metal Solution Component Recipe

Directions

For 1 L Total

  1. To approximately 950 mL of dH2O, add the nutrients in the order listed while stirring continuously.
    • Note: The Na2EDTA should be fully dissolved before adding other components.
  2. Bring total volume to 1 L with dH2O.
  3. Store in dark at freezer temperature.

Note final concentration listed is for the stock solution.


# Component Amount Stock Solution Concentration Final Concentration
1 Na2EDTA2H2O (Sigma ED255) 0.75 g/L 2 mM
2 FeCl36H2O (Sigma F-1513) 0.097 g/L 0.36 mM
3 MnCl24H2O (Baker 2540) 0.041 g/L 0.21 mM
4 ZnCl2 (Sigma Z-0152) 0.005 g/L 0.037 mM
5 CoCl26H2O (Sigma C-3169) 0.002 g/L 0.0084 mM
6 Na2MoO42H2O (J.T. Baker 3764) 0.004 g/L 0.017 mM

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.