|The final medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps.
For 1 L Total
1. To approximately 850 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
2. Bring the total volume to 1 L with dH2O.
3. Cover and autoclave medium.
4. When cooled add vitamins.
5. Store at refrigerator temperature.