Macro Component 2 for J Medium Recipe


Macro Component 2 for J Medium Recipe

Directions

For 1 L Total


1. To approximately 850 mL of dH2O, add the following components in the order listed while stirring continuously.
2. Adjust the pH to 7.5.
3. Bring the total volume to 1 L with dH2O.
4. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 Na2EDTAå2H2O (Sigma ED255) 4.4 g/L 0.12 mM
2 MgSO47H2O (Sigma 230391) 10 g/L 0.41 mM
3 MgCl26H2O (Fisher M 33) 5 g/L 0.24 mM
4 FeCl36H2O (Sigma F-1513) 0.5 g/L 0.02 mM

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.