Macro Component 1 for J Medium Recipe

Macro Component 1 for J Medium Recipe


For 1 L Total

  1. To approximately 900 mL of dH2O, add the following components in the order listed while stirring continuously.
  2. Adjust the pH 8.5 to 8.8.
  3. Bring the total volume to 1 L with dH2O.
  4. Store at refrigerator temperature.

Note final concentration listed is for the stock solution.

# Component Amount Stock Solution Concentration Final Concentration
1 NaNO3 (Fisher BP360-500) 67 g/L 7.9 mM
2 Na2HPO4 (Sigma S5136) 0.67 g/L 0.05 mM
3 K2HPO4 (Sigma P 3786) 3.8 g/L 0.22 mM
4 Tricine (Sigma T-5816-256) 25 g/L 1.4 mM

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.