G9 Trace Metals for J Medium Recipe


G9 Trace Metals for J Medium Recipe

Directions

For 1 L Total


1. To approximately 900 mL dH2O, add the following components in the order listed while stirring continuously.
2. Bring total volume to 1 L with dH2O.
3. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 H3BO3 (Baker 0084) 3.25 g/L 0.053 mM
2 MnSO4H2O (Sigma M8179) 1.5 g/L 8.6 µM
3 ZnSO47H2O (Sigma Z 0251) 0.3 g/L 1 µM
4 (NH4)6Mo70244H2O (Mallinckrodt 3420) 0.08 g/L 0.065 µM
5 CuSO45H2O (MCIB 3M11) 0.05 g/L 0.2 µM
6 Co(NO3)26H2O (Mallinckroft 4544) 0.15 g/L 0.52 µM
7 KI (Sigma P 4286) 0.01 g/L 0.06 µM

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.