Fe EDTA Recipe

Fe EDTA Recipe


For 1 L Total

  1. Dissolve Na2EDTA in 700 mL of dH2O.
  2. Adjust the pH to 7.5.
  3. Dissolve Fe(NH4)2(SO4)2.6H2O in 100 mL of dH2O.
  4. Add the two solutions together and bring the final volume to 1 L with dH2O.

Note final concentration listed is for the stock solution.

# Component Amount Stock Solution Concentration Final Concentration
1 Na2EDTA2H2O (Sigma ED255) 0.66 g/L 1.7 mM
2 Fe(NH4)2(SO4)26H2O (Sigma F-1513) 0.7 g/L 1.7 mM

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.