Enrichment Solution for Seawater Medium Recipe


Enrichment Solution for Seawater Medium Recipe

Directions

For 2 L Total

  1. To approximately 1 L of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
  2. Adjust the pH to 7.8.
  3. Bring the total volume to 2 L with dH2O.
  4. Cover and autoclave medium.
  5. When cooled add vitamins.
  6. Store at refrigerator temperature.

Note final concentration listed is for the stock solution.


# Component Amount Stock Solution Concentration Final Concentration
1 NaNO3 (Fisher BP360-500) 4.7 g/2 L 27.65 mM
2 Na2glycerophosphate5H2O (Sigma G 6501 ) 0.7 g/2 L 1.6 mM
3 ES Fe Solution 325 mL/2 L
4 P-II Metal Solution 325 mL/2 L
5 HEPES buffer (Sigma H-3375) 6.5 g/2 L 14 mM
6 Vitamin B12 3 mL/2 L
7 Biotin Vitamin Solution 3 mL/2 L
8 Thiamine Vitamin Solution 3 mL/2 L

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.