Chu Stock Solution Recipe


Chu Stock Solution Recipe

Directions

For 100 mL Total


1. Add each of the components in the order specified while stirring continuously.
2. Bring total volume to 100 mL with dH2O.
3. Dispense into 10 mL volumes and autoclave.
4. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 CaCl22H2O (Sigma C-3881) 10 mL 3.67 g/100 mL dH20 2.5 mM
2 MgSO47H2O (Sigma 230391) 10 mL 3.69 g/100 mL dH20 1.5 mM
3 K2HPO4 (Sigma P 3786) 10 mL 0.87 g/100 mL dH20 0.5 mM
4 NaNO3 (Fisher BP360-500) 10 mL 8.5 g/100 mL dH20 10 mM
5 Na2SiO39H2O (Sigma 307815) 10 mL 2.84 g/100 mL dH20 1 mM
6 Ferric Citrate Stock Solution for Chu 10 mL/100 mL
7 Chu Micronutrient Solution 10 mL/100 mL

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.