Chu Stock Solution Recipe


Chu Stock Solution Recipe

Directions

For 1L Total

  1. Add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O.
  3. Dispense into 10 mL volumes and autoclave.
  4. Store at refrigerator temperature.

Note final concentration listed is for the stock solution.


# Component Amount Stock Solution Concentration Final Concentration
1 CaCl22H2O (Sigma C-3881) 10 mL 3.67 g/100 mL dH20 2.5 mM
2 MgSO47H2O (Sigma 230391) 10 mL 3.69 g/100 mL dH20 1.5 mM
3 K2HPO4 (Sigma P 3786) 10 mL 0.87 g/100 mL dH20 0.5 mM
4 NaNO3 (Fisher BP360-500) 10 mL 8.5 g/100 mL dH20 10 mM
5 Na2SiO39H2O (Sigma 307815) 10 mL 2.84 g/100 mL dH20 1 mM
6 Ferric Citrate Stock Solution for Chu 10 mL/100 mL
7 Chu Micronutrient Solution 10 mL/100 mL

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.