This solution requires the combination of two liquid components:
Component A: 1. Pour approximately 450 mL dH2O into a beaker. Heat the beaker in the microwave oven to near boiling. 2. Add 10.0 g of Na2EDTA to the stirring beaker. 3. After the EDTA fully dissolves, bring the total volume to 500 mL with dH2O.
Component B: 1. Pour approximately 450 mL 0.1 M HCL into a beaker. 2. Add 0.81 g of FeC3.6H2O to beaker. 3. Bring the total volume to 500 mL with dH2O. Slowly add Component B to the heated Component A while stirring.
Stock Solution Concentration
Na2EDTA•2H2O (Sigma ED255)
10 g/500 mL
FeCl3•6H2O (Sigma F-1513)
0.81 g/500 mL
UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.