Chelated Iron Solution Recipe


Chelated Iron Solution Recipe

Directions

This solution requires the combination of two liquid components:


Component A:
1. Pour approximately 450 mL dH2O into a beaker. Heat the beaker in the microwave oven to near boiling.
2. Add 10.0 g of Na2EDTA to the stirring beaker.
3. After the EDTA fully dissolves, bring the total volume to 500 mL with dH2O.


Component B:
1. Pour approximately 450 mL 0.1 M HCL into a beaker.
2. Add 0.81 g of FeC3.6H2O to beaker.
3. Bring the total volume to 500 mL with dH2O.
Slowly add Component B to the heated Component A while stirring.

# Component Amount Stock Solution
Concentration
Final Concentration
1 Na2EDTA2H2O (Sigma ED255) 10 g/500 mL 26 µM
2 0.1M HCL 450 mL 45 mM
3 FeCl36H2O (Sigma F-1513) 0.81 g/500 mL 3 µM

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.