Algal Trace Elements Solution Recipe


Algal Trace Elements Solution Recipe

Directions

For 1 L Total


1. To approximately 950 mL of dH2O first dissolve EDTA, then add each of the components in the order specified while stirring continuously.
2. Bring total volume to 1 L with dH2O.
3. Cover and autoclave medium.
4. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 Na2EDTA2H2O (Sigma ED255) 4.36 g/L 110 µM
2 FeCl36H2O (Sigma F-1513) 1 g/L 40 µM
3 CuSO45H2O (MCIB 3M11) 0.001 g/L 0.04 µM
4 ZnSO47H2O (Sigma Z 0251) 0.022 g/L 0.77 µM
5 CoCl26H2O (Sigma C-3169) 0.012 g/L 0.5 µM
6 MnCl24H2O (Baker 2540) 0.18 g/L 9.1 µM
7 Na2MoO42H2O (J.T. Baker 3764) 0.022 g/L 0.99 µM
8 H2SeO3 (Sigma 211176) 0.001 g/L 0.077 µM
9 Na3VO4 (Sigma 450243) 0.002 g/L 0.11 µM

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.