A+ Trace Components Recipe


A+ Trace Components Recipe

Directions

For 1 L Total


1. To approximately 900 mL of dH2O add the components and stock solutions in the order specified while stirring continuously.
2. Bring total volume to 1 L with dH2O at refrigerator temperature, away from bright light.

# Component Amount Stock Solution
Concentration
Final Concentration
1 H3BO3 (Baker 0084) 3.43 g/L 55.5 mM
2 ZnCl2 (Sigma Z-0152) 0.0315 g/L 0.23 mM
3 MoO3(85%) 0.003 g/L 0.021 mM
4 Vitamin B12
(cyanocobalamin, (Sigma V-6629)
0.0004 g/L 0.3 µM
5 FeCl3•6H2O (Sigma F-1513) 1 mL/L 3.89 g/100 mL 0.14 mM
6 MnCl2•4H2O (Baker 2540) 1 mL/L 4.3 g/100 mL 0.22 mM
7 CuSO45H2O (MCIB 3M11) 1 mL/L 0.003 g/100 mL 0.00012 mM
8 CoCl26H2O (Sigma C-3169) 1 mL/L 0.012 g/100 mL 0.0005 mM

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.