A+ Medium

Upcoming Order Deadlines:

Orders for algal culture media placed after 5:00 PM CST on Wednesday, November 14th through Wednesday, November 28th, will not ship until Monday, December 3rd, 2018. There will be no outgoing shipments on November 26th, 2018. Please view more information here.




A+ Medium Recipe

 

Directions

For 1 L Total

Liquid Media:

  1. To approximately 900 mL of dH2O add the first 8 components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O.
  3. Cover and autoclave medium.
  4. Add filter sterilized A+ Trace Components.
  5. Allow to cool then store at refrigerator temperature.

Agar Media:

  1. To approximately 390 mL of dH2O add the first 8 components in the order specified while stirring continuously.
  2. Bring total volume to 500 mL with dH2O.
  3. In a separate container add 15 g of agar to 500 mL of dH2O (final 1.5% w/v).
  4. Cover and autoclave both solutions.
  5. In a water bath allow both solutions to cool to 45-50 °C.
  6. Add sterile Sodium Thiosulfate to agar solution and mix well.
  7. Add filter sterilized A+ Trace Components.
  8. Combine both agar and liquid solutions, mix well. Note the agar can solidify quickly.
  9. Allow to cool then store at refrigerator temperature.
# Component Amount Stock Solution Concentration Final Concentration
1 NaCl (Fisher S271-500) 18 g/L   0.308 M
2 MgSO4·7H2O (Sigma 230391) 5 g/L   0.02 M
3 Na2EDTA·2H2O (Sigma ED255) 10 mL/L 0.3 g/100 mL 0.08 mM
4 KCl (Fisher P 217) 10 mL/L 6 g/100 mL 8.05 mM
5 CaCl2·2H2O (Sigma C-3881) 10 mL/L 3.7 g/100 mL 2.52 mM
6 NaNO3 (Fisher BP360-500) 10 mL/L 10 g/100 mL 11.8 mM
7 KH2PO4 (Sigma P 0662) 10 mL/L 0.5 g/100 mL 0.37 mM
8 Trizma Base pH 8.2 10 mL/L 10 g/100 mL 8.26 mM
9 A+ Trace Components (sterilize before adding) 10 mL/L    
10 Sodium Thiosulfate Pentahydrate (agar media only,sterile) (Baker 3946) 1 mL/L 24.819 g/100 mL 1 µM