5/3 Soil + Seawater Agar Medium



5/3 Soil + Seawater Agar Medium Recipe

*Media available in 10-mL agar tubes only*

For marine algae that require salinities at 48 ppt.

Directions

For 1 L Total

  1. Filter 1 L of Soil + Seawater Medium to remove soil particles.
  2. Add 15 g of natural sea-salt in a stirring beaker wait until fully dissolved.
  3. Add 15 g of agar into the flask; do not mix.
  4. Cover and autoclave medium.
  5. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Soil + Seawater Medium 1 L
2 Natural Sea-salt 15 g