5% F/2 Medium



5% F/2 Medium Recipe

For marine algae that require salinities at 48 ppt.

Directions

For 1 L Total

  1. Dissolve 15 g of natural sea-salt in approximately 900 mL of non-pasteurized seawater (30-35 ppt).
  2. Add each of the components in the order specified (except vitamins) while stirring continuously.
  3. Bring total volume to 1 L with non-pasturized seawater.
    • *For 1.5% (w/v) agar medium: add 15 g of agar into the flask; do not mix.
  4. Cover and autoclave medium. 
  5. Add sterile vitamins to cooled medium.
    • *For agar medium add vitamins, mix, and dispense before agar solidifies. 
  6. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Seawater (non-sterilized) 1 L    
2 Natural Sea-salt 15 g/L    
3 NaNO3 (Fisher BP360-500) 1 mL/L 7.5 g/100 mL dH2O 880 µM
4 NaH2PO4·H2O(MCIB 742) 1 mL/L 0.5 g/100 mL dH2O 36 µM
5 Na2SiO3·9H2O (Sigma 307815) 1 mL/L 2 g/100 mL dH2O 70 µM
6 Trace Metals Solution 1 mL/L    
7 Vitamin B12 Solution 1 mL/L    
8 Biotin Vitamin Solution 1 mL/L    
9 Thiamine Vitamin Solution 1 mL/L