For marine algae that require salinities at 60 ppt. Add 1 L of pasteurized supplemented seawater divided into 200 mL volumes and combined with soil in preparation for pasteurizing with steam.
For 200 mL Total
|#||Component||Amount||Stock Solution Concentration||Final Concentration|
|1||Green House Soil||5 mL|
|2||CaCO3 (Fisher C 64)||1 mg||0.05 mM|
|3||Supplemented Seawater||200 mL|