For marine algae that require salinity at 10 ppt, the basic Enriched Seawater Medium is combined with sterile distilled water prior to inoculating the new culture, such that 2/3 Enriched Seawater Medium combines Enriched Seawater Medium and sterile distilled water 2:1.
For 1 L Total
|#||Component||Amount||Stock Solution Concentration||Final Concentration|
|1||Enriched Seawater Medium||665 mL|
|2||Sterile dH2O||335 mL|