1/3 Soil + Seawater Medium



1/3 Soil + Seawater Medium Recipe

The medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps.

Directions

For 200 mL Total

  1. Combine all components in order.
  2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95°C in 15 minutes. Then increased just over 98°C for the 3 hour duration. Cooling occurs gradually at room temperature.
  3. Refrigerate 24 hours or more and bring to room temperature before using.

# Component Amount Stock Solution Concentration Final Concentration
1 Green House Soil 1 tsp    
2 CaCO3 (optional) (Fisher C 64) 1 mg   0.05 mM
3 Pasteurized Seawater 67 mL    
4 dH2O 133 mL