1% F/2 Medium

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1% F/2 Medium Recipe

Directions

For 1 L Total

  1. Measure 285 mL of non-pasteurized seawater (30-35 ppt). 
  2. Add 600 mL of dH2O to bring the volume to approximately 900 mL. 
  3. Add each of the components in the order specified (except vitamins) while stirring continuously. 
  4. Bring up to 1 L total volume with Pasteurized Seawater. 
    • For 1.5% (w/v) agar medium: add 15 g of agar into the flask; do not mix.
  5. Cover and autoclave medium. 
  6. Add sterile vitamins to cooled medium.
    • For agar medium add vitamins, mix, and dispense before agar solidifies. 
  7. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Seawater (non-sterilized) 285 mL    
2 dH2O 600 mL    
3 NaNO3 (Fisher BP360-500) 1 mL/L 7.5 g/100 mL dH20 880 µM
4 NaH2PO4·H2O(MCIB 742) 1 mL/L 0.5 g/100 mL dH20 36 µM
5 Na2SiO3·9H2O (Sigma 307815) 1 mL/L 2 g/100 mL dH20 70 µM
6 Trace Metals Solution 1 mL/L    
7 Vitamin B12 1 mL/L    
8 Biotin Vitamin Solution 1 mL/L    
9 Thiamine Vitamin Solution 1 mL/L