1/5 Soil + Seawater Medium

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Orders for algal culture media placed after 5:00 PM CST on Wednesday, November 14th through Wednesday, November 28th, will not ship until Monday, December 3rd, 2018. There will be no outgoing shipments on November 26th, 2018. Please view more information here.

1/5 Soil + Seawater Medium Recipe

For some marine and brackish-water algae requiring salinities at 6 ppt, glass-distilled water and pasteurized, filtered seawater are combined prior to steaming, such that 1/5 Soil + Seawater contains seawater combined with distilled water 1:4.


For 200 mL Total

  1. Combine all components.
  2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
  3. Refrigerate (10 °C) 24 hours or more and bring to room temperature before using.

# Component Amount Stock Solution Concentration Final Concentration
1 Green House Soil 1 tsp    
2 CaCO3 (optional) (Fisher C 64) 1 mg   0.05 mM
3 Pasteurized Seawater 40 mL    
4 dH2O 160 mL