For some marine and brackish-water algae requiring salinities at 6 ppt, glass-distilled water and pasteurized, filtered seawater are combined prior to steaming, such that 1/5 Soil + Seawater contains seawater combined with distilled water 1:4.
For 200 mL Total
|#||Component||Amount||Stock Solution Concentration||Final Concentration|
|1||Green House Soil||1 tsp|
|2||CaCO3 (optional) (Fisher C 64)||1 mg||0.05 mM|
|3||Pasteurized Seawater||40 mL|