1/3 Soil + Seawater Medium

Upcoming Order Deadlines:

Orders for algal culture media placed after 5:00 PM CST on Wednesday, November 14th through Wednesday, November 28th, will not ship until Monday, December 3rd, 2018. There will be no outgoing shipments on November 26th, 2018. Please view more information here.




1/3 Soil + Seawater Medium Recipe

The medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps.

Directions

For 200 mL Total

  1. Combine all components in order.
  2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95°C in 15 minutes. Then increased just over 98°C for the 3 hour duration. Cooling occurs gradually at room temperature.
  3. Refrigerate 24 hours or more and bring to room temperature before using.

# Component Amount Stock Solution Concentration Final Concentration
1 Green House Soil 1 tsp    
2 CaCO3 (optional) (Fisher C 64) 1 mg   0.05 mM
3 Pasteurized Seawater 67 mL    
4 dH2O 133 mL