1/3 Soil + Seawater Medium Recipe
The medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps.
For 200 mL Total
- Combine all components in order.
- Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95°C in 15 minutes. Then increased just over 98°C for the 3 hour duration. Cooling occurs gradually at room temperature.
- Refrigerate 24 hours or more and bring to room temperature before using.
||Stock Solution Concentration
||Green House Soil
||CaCO3 (optional) (Fisher C 64)