For marine algae that require salinities at 15 ppt, the basic Enriched Seawater Medium is combined with sterile distilled water prior to inoculating the new culture, such that 1/2 Enriched Seawater Medium combines Enriched Seawater Medium and sterile distilled water 1:1.
For 1 L Total
|#||Component||Amount||Stock Solution Concentration||Final Concentration|
|1||Enriched Seawater Medium||500 mL|
|2||Sterile dH2O||500 mL|