UTEX 1444
Anabaena flos-aquae



Algae Details

UTEX Number: 1444
Class: Cyanophyceae
Strain: Anabaena flos-aquae
Medium: BG-11 Medium (BG-11)
Origin: Mississippi, USA
Description of Location: freshwater
GPS:
Type Culture: No
Collection:
Isolation: T. Tischer (1964)
Isolator Number: MSU A-37
Deposition: (9/65)
Relatives: CCAP 1403/13A; ATCC 29413; PCC 7937 Nostoc sp.; UTCC 67; UTCC B105; CCMEE 380; ACMM 485
Also Known As: A.variabilis (Healey 1973); Nostoc sp.
Notes: iron limitation, nitrogen fixation, siderophore production (Kerry et al. 1988); ferredoxin-NADP+ oxidoreductase, NADPH dehydrogenase (Scherer et al. 1988); amino acid biosynthesis (Niven et al. 1988a); amino acid liberation (Niven et al. 1988b); contains restriction endonuclease Avr (Streiner 1990); (Tischer & Moore 1964, Duyvesteyn et al. 1983); grown in shaded light

General Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.


Preparation of Living Algal Strains For Orders

For agar cultures, strains are transferred to the surface of fresh agar slants in 20 mm diamter glass screw-cap tubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears.

Agar and liquid cultures are packaged within a Styrofoam box and shipped the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.


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